e2f3 sirna Search Results


si03650318 e2f3 sirna horizon discovery  (Thermo Fisher)
92
Thermo Fisher si03650318 e2f3 sirna horizon discovery
Si03650318 E2f3 Sirna Horizon Discovery, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/si03650318 e2f3 sirna horizon discovery/product/Thermo Fisher
Average 92 stars, based on 1 article reviews
si03650318 e2f3 sirna horizon discovery - by Bioz Stars, 2026-02
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e2f3 sirna  (Santa Cruz Biotechnology)
92
Santa Cruz Biotechnology e2f3 sirna
Figure 3. Both E2F1 and <t>E2F3</t> contribute to E1A-induced apoptosis and p53 induction. (A) Serum-starved wildtype (Wt) and E2f1-/- MAFs were infected with AdCMV empty vector or AdE1A at MOI of 100. 48 h post-infection caspase 3 activity was measured with the fluorescence substrate Ac-DEVD-AFC and the average of three experiments is presented. Bars indicate standard error and *indicates statistical significance compared to E1A-infected wildtype MAFs (p < 0.05). (B) The same cells were treated as above or exposed to 10 Gy of IR one h prior to harvest. Western blot analysis was performed on protein lysates (50 μg) using antibodies to phospho-serine 15 p53, total p53, E1A and β-actin as indicated. (C) Primary NHFs were transfected with control <t>siRNA</t> or siRNA directed to E2F3 under starvation conditions and 24 h later infected with AdCMV or AdE1A at MOI of 100. 48 h post-infection caspase 3 activity was measured with the fluorescence substrate Ac-DEVD-AFC and the average of three experiments is presented. Bars indicate standard error and *indicates statistical significance compared to E1A-infected NHFs (p < 0.05). (D) NHFs were transfected with control (lanes 1 and 2) or E2F3 (lanes 3 and 4) siRNA and then infected with AdCMV (lanes 1 and 3) or AdE1A (lanes 2 and 4) as described above. Western blot analysis was performed on protein lysates (50 μg) using antibodies to E2F3, E2F1, phospho-serine 15 p53, γH2AX and H2B as indicated.
E2f3 Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/e2f3 sirna/product/Santa Cruz Biotechnology
Average 92 stars, based on 1 article reviews
e2f3 sirna - by Bioz Stars, 2026-02
92/100 stars
  Buy from Supplier

Image Search Results


Figure 3. Both E2F1 and E2F3 contribute to E1A-induced apoptosis and p53 induction. (A) Serum-starved wildtype (Wt) and E2f1-/- MAFs were infected with AdCMV empty vector or AdE1A at MOI of 100. 48 h post-infection caspase 3 activity was measured with the fluorescence substrate Ac-DEVD-AFC and the average of three experiments is presented. Bars indicate standard error and *indicates statistical significance compared to E1A-infected wildtype MAFs (p < 0.05). (B) The same cells were treated as above or exposed to 10 Gy of IR one h prior to harvest. Western blot analysis was performed on protein lysates (50 μg) using antibodies to phospho-serine 15 p53, total p53, E1A and β-actin as indicated. (C) Primary NHFs were transfected with control siRNA or siRNA directed to E2F3 under starvation conditions and 24 h later infected with AdCMV or AdE1A at MOI of 100. 48 h post-infection caspase 3 activity was measured with the fluorescence substrate Ac-DEVD-AFC and the average of three experiments is presented. Bars indicate standard error and *indicates statistical significance compared to E1A-infected NHFs (p < 0.05). (D) NHFs were transfected with control (lanes 1 and 2) or E2F3 (lanes 3 and 4) siRNA and then infected with AdCMV (lanes 1 and 3) or AdE1A (lanes 2 and 4) as described above. Western blot analysis was performed on protein lysates (50 μg) using antibodies to E2F3, E2F1, phospho-serine 15 p53, γH2AX and H2B as indicated.

Journal: Cell cycle (Georgetown, Tex.)

Article Title: E2F1 and E2F3 activate ATM through distinct mechanisms to promote E1A-induced apoptosis.

doi: 10.4161/cc.7.3.5286

Figure Lengend Snippet: Figure 3. Both E2F1 and E2F3 contribute to E1A-induced apoptosis and p53 induction. (A) Serum-starved wildtype (Wt) and E2f1-/- MAFs were infected with AdCMV empty vector or AdE1A at MOI of 100. 48 h post-infection caspase 3 activity was measured with the fluorescence substrate Ac-DEVD-AFC and the average of three experiments is presented. Bars indicate standard error and *indicates statistical significance compared to E1A-infected wildtype MAFs (p < 0.05). (B) The same cells were treated as above or exposed to 10 Gy of IR one h prior to harvest. Western blot analysis was performed on protein lysates (50 μg) using antibodies to phospho-serine 15 p53, total p53, E1A and β-actin as indicated. (C) Primary NHFs were transfected with control siRNA or siRNA directed to E2F3 under starvation conditions and 24 h later infected with AdCMV or AdE1A at MOI of 100. 48 h post-infection caspase 3 activity was measured with the fluorescence substrate Ac-DEVD-AFC and the average of three experiments is presented. Bars indicate standard error and *indicates statistical significance compared to E1A-infected NHFs (p < 0.05). (D) NHFs were transfected with control (lanes 1 and 2) or E2F3 (lanes 3 and 4) siRNA and then infected with AdCMV (lanes 1 and 3) or AdE1A (lanes 2 and 4) as described above. Western blot analysis was performed on protein lysates (50 μg) using antibodies to E2F3, E2F1, phospho-serine 15 p53, γH2AX and H2B as indicated.

Article Snippet: NHFs were plated at 4,000 cells/cm2 in 6 cm plates and allowed to recover overnight in complete media, 10% FBS DMEM with antibiotics and transfected with 200–250 pmol of E2F3 siRNA or control siRNA (Santa Cruz biotechnology, Inc.) using Oligofecatamin reagent (invitrogen) according to the manufacturer’s protocol.

Techniques: Infection, Plasmid Preparation, Activity Assay, Fluorescence, Western Blot, Transfection, Control

Figure 6. DNA breaks induced by E1A are unaffected by loss of E2F1 but reduced by knockdown of E2F3. (A) Wildtype (Wt) and E2f1-/- MAFs were infected with AdCMV empty vector or AdE1A at MOI of 100. 48 h post-infection cells were subjected to the comet assay and analyzed as previously described. Bars indicate standard error and *indicates statistical significance compared to AdCMV infected cells of the same genotype (p < 0.05). (B) NHFs were transfected with control siRNA or E2F3 siRNA 24 h prior to infections with AdCMV or AdE1A at MOI of 100. 48 h post-infection cells were subjected to the comet assay and analyzed as previously described. Bars indicate standard error and *indicates statistical significance compared to control siRNA treated cells infected with AdE1A.

Journal: Cell cycle (Georgetown, Tex.)

Article Title: E2F1 and E2F3 activate ATM through distinct mechanisms to promote E1A-induced apoptosis.

doi: 10.4161/cc.7.3.5286

Figure Lengend Snippet: Figure 6. DNA breaks induced by E1A are unaffected by loss of E2F1 but reduced by knockdown of E2F3. (A) Wildtype (Wt) and E2f1-/- MAFs were infected with AdCMV empty vector or AdE1A at MOI of 100. 48 h post-infection cells were subjected to the comet assay and analyzed as previously described. Bars indicate standard error and *indicates statistical significance compared to AdCMV infected cells of the same genotype (p < 0.05). (B) NHFs were transfected with control siRNA or E2F3 siRNA 24 h prior to infections with AdCMV or AdE1A at MOI of 100. 48 h post-infection cells were subjected to the comet assay and analyzed as previously described. Bars indicate standard error and *indicates statistical significance compared to control siRNA treated cells infected with AdE1A.

Article Snippet: NHFs were plated at 4,000 cells/cm2 in 6 cm plates and allowed to recover overnight in complete media, 10% FBS DMEM with antibiotics and transfected with 200–250 pmol of E2F3 siRNA or control siRNA (Santa Cruz biotechnology, Inc.) using Oligofecatamin reagent (invitrogen) according to the manufacturer’s protocol.

Techniques: Knockdown, Infection, Plasmid Preparation, Single Cell Gel Electrophoresis, Transfection, Control